Comparative assessment of phenolic composition profile and biological activities of green extract and conventional extracts of Salvia sclarea

In recent years, there have been an attempt to develop safe and environmental friendly solvents to replace conventional solvents, and use for extraction bioactive compounds from natural sources. A current investigation involved the preparation of green, methanolic, and ultrasonic extracts of S. sclarea, and compared their phenolic profiling using HPLC–DAD, antibacterial, antioxidant, and enzyme inhibition activities. The HPLC–DAD analysis revealed that Rosmarinic acid was the main content in all extracts, with Ellagic acid only present in the green extract. The green extract exhibited superior anti-biofilm activity against S. Aureus and E. Faecalis compared to the other extracts at MIC concentration. Furthermore, the green extract also displayed the highest inhibition of swarming motility in P. Aeruginosa with inhibition range 68.0 ± 2.1 (MIC) to 19.5 ± 0.6 (MIC/4). and better enzyme inhibitory activity against BChE (with IC50 = 131.6 ± 0.98 µg/mL) and AChE (with inhibition 47.00 ± 1.50%) compared to the other extracts; while, the ultrasonic extract showed strong inhibition of violacein production by C. Violaceum with a inhibition range 05.5 ± 0.1 (MIC/32) to 100 ± 0.00 (MIC), followed by the green extract with a inhibition range 15.0 ± 0.5 (MIC/8) to 100 ± 0.00 (MIC), additionally, the ultrasonic and methanoic extracts showed significant activity against urease enzyme with (IC50 = 171.6 ± 0.95 µg/mL and IC5 0 = 187.5 ± 1.32 µg/mL) respectively. Both the green and methanolic extracts showed considerable antioxidant activities, as β-carotene-linoleic acid (IC50 = 5.61 ± 0.47 µg/mL and 5.37 ± 0.27 µg/mL), DPPH· (IC50 = 19.20 ± 0.70 µg/mL and 16.31 ± 0.23 µg/mL), ABTS·+(IC50 = 8.64 ± 0.63 µg/mL and 6.50 ± 0.45 µg/mL) and CUPRAC (A0.5 = 17.22 ± 0.36 µg/mL and 12.28 ± 0.12 µg/mL) respectively, likewise the green extract performing better in metal chelating compared to the other extracts. The green extraction is reported as a cost effective and solvent free method for extracting natural products that produces compounds free of toxic chemicals. This could be the method to be used in the industries as a renewable method.


Extraction procedures
Methanol extraction Exact (10 g) of S. sclarea (aerial parts) were soaked in 300 mL of methanol at room temperature for 72 h.The mixture was then filtered through a cellulose filter paper with a pore size of 0.45 μm.The methanol was removed by evaporation under reduced pressure using a rotary evaporator (Heidolph, Hei-Vap Value Gl, Germany), resulting in the formation of a dry extract with a brownish-green gummy consistency.

Ultrasonic assisted extraction
Ultrasonic cleaner, [model lab.Ult.4030) comprised of a processor unit Q600 (500 W, 40 kHz)] was used for extraction.(10 g) of S. sclarea (aerial part) were transferred into a 500 mL conical beaker, 300 mL of methanol was added, the beaker was sealed, and the extraction was performed for 25 min.After extraction, the solution was filtered by using a filter paper (0.45 μm cellulose) and the extract was concentrated by using rotary evaporator.

Green extraction
Preparation of natural deep eutectic solvents (NADES).Green extraction of S. sclarea was carried out using natural deep eutectic solvent (NADES) (citric acid: glucose) was prepared following the protocol described previously 17,18 .Briefly, citric acid and glucose were mixed at molar ratio (1:2) and located into sealed conical flask 250 mL; the mixture was subjected to constant string and heated to a temperature of 80 °C until homogeneous liquid was achieved.
Crude extraction using green solvent.Extraction was performed by adding the green solvent to 3 g of plant material in a sealed conical flask, the; distilled water (20 mL) was also added to lower the viscosity.The mixture was heated to a temperature of 40 °C and stirred for a duration of 10 h.Afterward, the sample was transferred into a falcon tube with a capacity of 50 mL.The falcon tube was then subjected to centrifugation at a speed of 4000 for a period of 20 min.The resulting sediments were discarded, and the remaining liquid portion (supernatant) was filtered using a cellulose paper with a pore size of 0.45 μm.Following filtration, the aqueous mixture underwent evaporation using a rotary evaporator, followed by freeze-drying.Finally, the freeze-dried product was stored at a temperature of 30 °C for additional investigation. www.nature.com/scientificreports/

Determination of minimum inhibitory concentrations
For determination of the MICs, a microliter broth dilution method recommended by Clinical Laboratory Standards Institute was followed 20 .The test medium used was Mueller-Hinton broth and the bacterial density was 5 × 10 5 colony-forming units (CFU)/mL.The MIC was defined as the lowest compound or extract concentration that showed no visible growth.Inoculation of cell suspensions (100 μL) was done on 96-well microliter plates containing either extract(s) or varied concentrations (1, 0.5, 0.25, 0.125, 0.0625, 0.0312 mg/mL).The microplates were incubated at 37 °C and read after 24 h.

Effect of extract on bacterial biofilm formation
To examine the effect of S. sclarea extract(s) on bacterial biofilm formation, a microplate biofilm assay was conducted using a range of extract concentrations, including 1, 1/2, 1/4, and 1/8 MIC 21 .Briefly, overnight cultures of the test microorganisms were diluted to 1% and added to 200 μL of fresh Tryptose-Soy Broth (TSB) supplemented with 0.25% glucose, and then incubated in the absence and presence of extract(s) without agitation for 48 h at 37 °C.Wells containing only TSB and cells were used as controls.After incubation, the wells were washed with water to eliminate planktonic bacteria, and the remaining bacteria were stained with a 0.1% crystal violet solution for 10 min at room temperature.The wells were washed again to eliminate excess crystal violet, and 200 μL of 33% glacial acetic acid in ethanol was added to each well.The resulting solution was shaken and pipetted, and then transferred (125 μL) to a sterile tube.The volume was adjusted to 1 mL with distilled water.Finally, optical density (OD) of each well was measured at wavelength of 550 nm using a Thermo Scientific Multiskan FC instrument (Vantaa, Finland).The percentage of inhibition caused by the tested extracts was calculated using the following formula:

Bioassay for quorum-sensing inhibition (QSI) activity using C. violacium CV026
In order to assess the quorum-sensing inhibition (QSI) activity of S. sclarea extract(s), a bioassay using C. violacium CV026 was conducted with some modifications as described elsewhere 22 with small adjustments.Five milliliters of warm molten Soft Top Agar (consisting of 2.0 g tryptone, 1.3 g agar, 200 mL deionized water and 1.0 g sodium chloride) was seeded with 100 μL of overnight C. violacium CV026 culture, followed by the addition of 20 μL of 100 μg/mL C 6 HSL as an exogenous AHL source.The mixture was gently mixed and immediately poured over the surface of a solidified LBA plate as an overlay.Wells with a diameter of 5 mm were made on each plate after the overlay had solidified.All well was then occupied with 50 μL of sub-MIC concentrations of filtersterilized extract.The development of a white or cream-colored halo around the well against a purple lawn of activated C. violacium CV026 bacteria indicated QSI, the antimicrobial activity of the extracts was determined by observing the clear halo around the samples.To determine the limit of detection, serial dilutions of the extracts were made using LB broth as a diluent, ranging from (1:1 to 1:8).The end points were determined as the lowest dilution of extract that showed noticeable inhibition of violacein synthesis.Each experiment was repeated three times and the assay plates were incubated at 30 °C for 3 days.After incubation, the diameters of the quorum sensing inhibition zones were measured.

Violacein inhibition assay using C. violacium CV12472
Qualitative analysis was conducted on extracts of S. sclarea to determine their potential as quorum sensing inhibitors (QSI) against C. violaceum ATCC 12472 23 .An overnight culture of C. violaceum (adjusted to 0.4 OD) was added to sterile microliter plates containing 200 μL of LB broth.The plates were then incubated in the presence and absence of sub-MICs of the extract.A positive control was included using LB broth containing C. violaceum ATCC 12472.The plates were incubated at 30 °C for 24 h and observed for a decrease in violacein pigment production, with absorbance readings taken at 585 nm.The percentage of violacein inhibition was calculated using the following formula: Swarming motility inhibition on P. aeruginosa PA01 The inhibition of swarming motility assay by S. sclarea extract(s) was conducted according to a previously described method 24 .In summary, P. aeruginosa PAOI strain overnight cultures were point inoculated at the centre of swarming plates containing of 0.5% NaCl, 1% peptone, 0.5% filter-sterilized d-glucose and 0.5% agar.Different concentrations of extract (50, 75, and 100 μg/mL) were added to the plates, while a plate without extract served as the control.The plates were then incubated upright at an appropriate temperature for 18 h.The migration of swarming was observed by tracking the movement of bacterial cells at the swarm fronts.

Anticholinesterase activity
The extracts of S. sclarea was tested for its anticholinesterase activity by determining its inhibitory effects on acetylcholinesterase and butyrylcholinesterase using a spectrophotometer and following the protocol described by Ellman with slight modifications 25 .The reference compound used was Galantamine, and the IC 50 values were calculated using a program derived from the anticholinesterase graph.The inhibitory activity was expressed as percentages (% inhibition) relative to sample concentrations (μg/mL).

Anti-urease activity
To evaluate the inhibitory activity of urease enzyme by each extract, ammonia production was determined using the indophenol method with a microplate reader 26 .In brief, 25 μL of enzymatic urease solution obtained from jack bean source, 50 μL of 100 mM Urea, and 100 mM Sodium phosphate buffer (pH 8.2) were mixed and incubated at 30 °C for 15 min after adding the sample (10 μL extracts).Then, 45 μL of Phenol reagent and 70 μL of Alkali reagent were added to each well.After 50 min of incubation, the absorbance was recorded at wavelength of 630 nm using a microplate reader.Thiourea was used as the reference compound, and the results were expressed as a 50% inhibitory concentration (IC 50 ).

Antioxidant activities
Antioxidant properties of all extracts were assessed using various methods, which included: (1) β-carotenelinoleic acid assay, (2) DPPH assay, (3) ABTS assay, (4) CUPRAC assay and (5) metal chelating assay.Inhibition of lipid peroxidation activity was performed by using β-carotene-linoleic acid test system according to the standards protocol developed 27 with minor modifications 28 .The DPPH assay was performed by using a spectrophotometer according to typical methods documented previously 29,30 .The ABTS + assay was carried out as described previously by Marco, 1968 31 with slight modification 32 .The cupric reducing antioxidant capacity (CUPRAC) was evaluated by following the method published previously 33 .α-Tocopherol and butylated hydroxyanisole (BHA) were used as antioxidant standards to compare the DPPH, β-carotene-linoleic acid, CUPRAC and ABTS + assays.The metal chelating assay of extracts for Fe 2+ was performed by using a spectrophotometer as the method reported previously 34 .The standard compound used was ethylenediaminetetraacetic acid (EDTA).The results of metal chelating assay were given as the % inhibition of 200 mg/mL concentration.

Statistical analysis
The activities were performed three times each, and the mean ± standard error of the mean was recorded for the results.To determine significant differences between means, Student's test was utilized with a significance level of 0.05.

Ethical approval
It is to confirm that all the methods described in the manuscripts are in accordance with the relevant guideline and regulations, as and when needed they are cited accordingly.

Results
The analysis and comparison of polyphenol compounds using HPLC-DAD and study their biological activities were conducted on all three extracts obtained which include methanol, ultrasonic extraction and green extraction.

Phenolic compositions
The phenolic composition of three types of extracts of S. sclarea including; methanolic extract, ultrasonic extract, and green extract, were determined and quantified in μg/g by using HPLC-DAD.Twenty-six reference phenolic compounds as standards were used.The results are reported in Table 1, showing that the Rosmarinic acid and Quercetin were in high quantity in all extracts, in ultrasonic extract both were quantified as 191.www.nature.com/scientificreports/ in green extract both phenolic were found as 90.31 ± 0.36 μg/g and 4.41 ± 0.25 μg/g, respectively.The phenolic compounds including; Gallic acid, Catechin, Caffeic acid, Syringic acid, Coumarin, Rutin, Rosmarinic acid, Myricetin, Quercetin, Luteolin and Apigenin were found in methanolic, ultrasonic and green extract of S. sclarea in variable quantities.While; 3-Hydroxy benzoic acid, Syringic acid, Ferulic acid, and Trans-cinnamic acid were only found in methanolic and ultrasonic extracts, whereas the Ellagic acid (3.40 ± 0.11 μg/g) was present only in green extract.The Protocatechuic acid, Pyrocatechol, Chlorogenic acid, P-hydroxy benzoic acid, and 6,7-Dihydroxy coumarin were not detected in any of the extract.The HPLC-DAD chromatograms of methanolic extract, ultrasonic extract, and green extract of S. sclarea are shown in Fig. 1.The structures of identified compounds in extracts were given in Fig. 2 Biological activity

Minimum inhibitory concentration (MIC)
The minimum inhibitory concentration (MIC) of green extract compared to methanol and ultrasonic extracts of S. sclarea were evaluated against E. coli, E. faecalis, C. albicans, S. aureus and P. aeruginosa PA01 and the results are presented in Table 2 and Fig. 3.The methanolic extract of S. sclarea showed better activity with MIC value 0.5 mg/mL than ultrasonic and green extract on E. coli (with MIC value 2.5 mg/mL and 2.5 g/mL respectively), E. faecalis (with MIC value 0.625 mg/mL and 1.25 m/mL respectively) and P. aeruginosa PA01 (with MIC value 2.5 g/mL), while ultrasonic extract and green extract showed similar activity on C .albicans and P. aeruginosa PA01 with MIC value 2.5 mg/mL and 1.25 g/mL respectively.However, the green extract of S. sclarea showed an overall better inhibitory effect than the methanolic extract and ultrasonic extract against S. aureus strain with MIC value 0.625 mg/mL.Chromobacterium is a model organism used for measuring the quorum-sensing inhibition potential of bacteria.This is because it produces the bis-indole alkaloid pigment called violacein whose colour is easily measurable 35 .Using the C. violaceum CV12472, the concentration dependent decrease in violacein (purple) coloration reflects the inhibition of violacein which is a quorum sensing mediated process.The violacein plays the role of a signal molecule which aids bacteria cell-to-cell communication within colonies for coordinated behavior 36 .The extracts inhibited violacein inhibition and the percentage inhibition decreased with decreasing concentrations from MIC towards lower sub-MICs.This is reflected by the increasing violacein pigmentation down the plate as shown on Fig. 4. The mutant strain C. violaceum CV026 does not produce violacein except when an external acylhomoserine lactone is supplied to it.Chemical substances which can prevent this bacterium from producing the purple coloration in the presence of supplied acylhomoserine lactone hormone are known www.nature.com/scientificreports/ to possess anti-quorum sensing activity 37,38 .This is reflected by the brownish halos around the wells against the purple lawn as shown on Fig. 4, reflecting inhibition zones whose diameters are measured in millimeters.

Percentage biofilm inhibition
The biofilm inhibition of each extract against selected pathogens was evaluated at MIC and sub-MIC concentrations as described in  4. The minimum inhibitory concentration (MIC) of the selected strain against each extract was measured prior, and the quorum sensing inhibition at MIC and sub-MIC concentrations were determined.The MIC values of methanolic, ultrasonic and green extracts of S. sclarea against C. violaceum CV026 was recorded as 0.5 mg/mL, 0.25 mg/mL and 0.25 mg/mL respectively.Among the extracts tested, the ultrasonic extract showed a highest anti-quorum sensing activity with an inhibition zone of 15.5 ± 0.5 mm, 12.5 ± 0.2 mm, and 10.0 ± 0.1 mm at MIC, MIC/2 and MIC /4 concentration in turn, while the green extract showed slightly anti quorum sensing activity with an inhibition zone of 14.0 ± 1.1 mm, 12.0 ± 0.4 mm and 9.5 ± 0.5 mm at MIC, MIC/2 and MIC /4 concentrations in turn.The least anti-quorum sensing activity was recorded for methanolic extract with an inhibition zone of 13.5 ± 1.0 mm and 10.0 ± 0.5 mm at Consequently, quorum sensing inhibition potential were recorded as ultrasonic extract > green extract > methanolic extract as shown in Fig. 5.

Inhibition of violacein production
The green extract, methanolic extract and ultrasonic extract were tested for antimicrobial activity using C. violaceum CV12472 to measure the inhibition of violacein production.The results reported in Table 5 and Fig. 6, all the extracts showed 100% violacein inhibition activity against C. violaceum CV12472 at MIC values as 1.25 mg/ mL, 0.625, 0.3125 mg/mL, respectively.The ultrasonic extract maintained 100% violacein inhibition even at MIC/2 concentration.Further, the percentage inhibition of violacein production by ultrasonic extract at sub-MIC concentration exhibited inhibition as 65.7 ± 1.5, 30.9 ± 1.8 and 05.5 ± 0.1 at MIC/4, MIC/8, MIC/16 and MIC/32.While, the percentage inhibition of violacein production by the green extract was 52.5 ± 2.5, 28.9 ± 1.2 and 15.0 ± 0.5 at MIC/2, MIC/4 and MIC/8, respectively.Likewise, the methanolic extract also showed percent inhibition of violacein production of 49.1 ± 0.9, 33.4 ± 1.1 and 20.6 ± 0.5 at a concentration of MIC/2, MIC/4, and MIC/8.Consecutively, hence the order of violacein production inhibition potential will be as ultrasonic extract > green extract > methanolic extract.

Swarming motility inhibition
The methanolic, ultrasonic and green extracts of S. sclarea were tested for swarming motility inhibition against the P. aeruginosa PA01 at MIC, MIC/2, and MIC/4 concentrations, as displayed in Table 6.The green extraction showed significant swarming motility inhibition compared to methanolic and ultrasonic extracts under similar conditions.The inhibition of swarming motility by green extraction was 68.0 ± 2.1%, 39.6 ± 0.8% and 19.5 ± 0.6% at MIC, MIC/2 and MIC/4 concentrations, respectively.While; the inhibition of swarming motility by the methanolic extract was 51.3 ± 1.8%, 24.8 ± 0.9% and 11.5 ± 0.4% at MIC, MIC/2 and MIC/4 concentrations, respectively.However, the inhibition of swarming motility by the ultrasonic extract was 44.9 ± 0.5%, 23.2 ± 1.2% and 06.2 ± 0.1% at concentrations of MIC, MIC/2 and MIC/4, respectively.Consequently, the order of the inhibition efficacy was as green extracts > methanolic extract > ultrasonic extract, as shown in Fig. 7.

Anticholinesterase activity
The ability of the methanolic, ultrasonic, and green extracts of S. sclarea to inhibit certain selected enzymes, including acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) were evaluated in vitro and the obtained results are displayed in Table 7. Very moderate percentage inhibition by each tested extract was recorded against AChE.Maximum AChE inhibition 47.00 ± 1.50% was shown by green extract, followed by methanolic Consequently, potential inhibition of AChE and BChE could be in order as green extracts > methanolic extract > ultrasonic extract, Figs. 8 and 9.
Anti-urease activity Table 7 presents the ability of methanolic, ultrasonic and green extracts of S. sclarea to inhibit the urease enzyme.

Antioxidant activities β-Carotene-linoleic acid assay
The inhibition of lipid peroxidation activity of methanol extract, green extract and ultrasonic extracts of S. sclarea were evaluated, and the results are presented in Table 8.The green and methanolic extracts exhibited potent activity with IC 50 (5.61± 0.47 µg/mL) and (5.37 ± 0.27 µg/mL) respectively; while ultrasonic extract showed appreciable activity with IC 50 value 36.58 ± 0.63 µg/mL.The activity of methanolic extract and the green extract was relatively close to each other and to the inhibition exhibited by standard BHA (IC 50 = 1.46 ± 0.03 μg/mL), and α-Tocopherol (IC 50 = 2.25 ± 0.04 μg/mL).

Metal chelating assay
The green extract, methanol extract and ultrasonic extract of S. sclarea were tested for antioxidant activity by using metal chelating assay and the results are presented in Table 8.Among of the tested extracts, the green extract exhibited highest metal chelating activity with IC 50 = 42.35 ± 1.04 µg/mL, followed by methanolic extract with IC 50 = 47.22 ± 0.54 µg/mL while the ultrasonic extract showed minimal antioxidant activity with IC 50 = 80.91 ± 1.20 µg/mL.For comparison the antioxidant activities of all extract(s) as tested by different methods is depicted in Fig. 11.

Discussion
This study was designed to analyze the presence of various phenolic compounds of S. sclarea by extraction through the green extraction process and its comparison with traditional methanol extraction and ultrasonic extraction.The phenolic compounds were abundant in all the tested extracts with variation in their quantities.
In the methanolic and ultrasonic extracts fourteen phenolic compounds including 3-Hydroxy benzoic acid, Syringic acid and Trans-cinnamic acid were exclusively available were not present in green extract.Likewise, in the green extract, 12 compounds were detected one of them was Ellagic acid, that was not found in other extracts.Phenolic compounds are secondary metabolites that contain benzene rings, with one or more hydroxyl groups, and range from simple phenolic molecules to highly polymerized compounds 39 .The phenolic compound plays an important role as an antioxidant agent, providing protection to the cells of organisms against oxidative damage 40 .Furthermore, phenolic compounds have the efficacy of different therapeutic properties such as antiaging, anti-inflammatory, anti-diabetic and anti-proliferative agents 41 .
The results of antibacterial activity against the selected bacteria strains, including S. aureus, E. coli, C. albicans, E. faecalis, and P. aeruginosa PA01 showed appreciable activity of methanolic, ultrasonic and green extracts of S. sclarea.
This forced us to evaluate further the potential inhibition of selected extracts toward bacterial biofilm formation.The biofilms are microbial communities, in which bacterial cells are embedded in a self-generated matrix of lipids, exopolysaccharides (EPS), proteins, and nucleic acids.This matrix blocks the entry of antimicrobial agents into cells 42,43 , hence providing the resistance to many bacteria against numerous drugs 44 .The results of Table 8.Antioxidant activity of the extracts of S. sclarea by the β-carotene-linoleic acid, DPPH • , ABTS •+ , CUPRAC, and metal-chelating assays a .NT not tested.a IC 50 values represent the means ± SEM of three parallel measurements (p < 0.05).b A 0.50 values represent the means ± SEM of three parallel measurements (p < 0.05).c Reference compounds.this study proved that the methanolic, ultrasonic and green extracts had inhibited the biofilm figuration, where methanolic extract showed inhibition against figuration of biofilm of four types of microbial strains including S. aureus, E. coli, C. albicans and E. faecali, similarly, the green extract showed good inhibition for figuration biofilm of four of bacteria strains that were S. aureus, E. coli, E. faecalis and P. aeruginosa PA01, while ultrasonic extract exhibited affection against biofilm formation of three of bacteria strains were S. aureus, E.coli and P. aeruginosa PA01.The observed results may be attributed to the destabilization of the cell membrane which could be caused by phenolic compounds present at concentrations below the MIC 45 .Additionally, the studies reported that formation of biofilm depends on several factors, including quorum sensing signaling 46 .However, detection of quorum inhibition is likely played a critical role in blocking biofilm formation.Further, studies are needed to assess the mechanisms of biofilm inhibition observed in this study and many others.Many researchers have also reported the inhibition of anti-biofilm formation.The methanol extract of Capparis spinosa (capers) inhibited the formation of biofilm biomass in P. mirabilis, E. coli, and P. aeruginosa PAO1 47 .Quorum sensing indicates to the connection system of bacteria at the molecular level where the bacteria react to different environmental signals and perform assignments as a group such as; virulence gene expression, biofilm formation, migration to more favorable environments, bacteriocin, antibiotic production and pigment production 48,49 .
A disarrangement in any of the steps needed for the quorum sensing communication could lead to overlap with microbial pathogenesis and help in bacterial control 50 .In the current study, all tested extracts showed inhibition of quorum sensing, the order of ability inhibition was as ultrasonic extract > green extract > methanolic extract.The Phenolic compounds were reported previously as anti-quorum sensing agent 51 , furthermore, numerous investigations demonstrated the capacity of phenolic compounds as an excellent inhibitor of violacein production.The studies found Caffeic acid reduced violazine production by up to 75%, Gallic acid by up to 59%, Oleuropine glycoside to 51% and Epicatechin 33%, 51%.Ferric acid by up to 72%, Floridine up to 48% 52,53 .In the current investigation, all extracts exhibited significant violacein production inhibition and the ultrasonic extract showed the highest inhibition, on the other hand, the phenolic compounds including Caffeic acid and Gallic acid identified in all extracts tested, therefore we hypothesize that the inhibition of violacein production observed in this investigation related to the presence of phenolic compounds acting individually or together in synergistically effect.Swarm motility is a quick and harmonious, translocation of a bacterial population to solid or semi-solid surfaces and is an example of bacterial multicellularity and swarm behaviour 54 .Bacterial swimming motility affects many pathogen-host interactions, and several pathogens are also capable of migrating multicellular swarms.The bacterial motility of swimming may be related to biofilm formation 55 .The phenolic compounds documented earlier as swarming motility inhibitors, for instance, the study by Borges et al. had exhibited inhibition capability of phenolic compounds including Gallic acid, Caffeic acid and Ferulic acid against swarming motility of P. aeroginosas, S. aureus, E. coli, and L. monocytogenes 52,56 , their results proved the significant potential of these phenolic compounds as swarming motility inhibitors agent.In the current study, as mentioned above the gallic acid, caffeic acid and ferulic acid were detected in all extracts tested, therefore, the potential inhibition swarming motility that have shown by extracts in our results may be linked to the existence of the phenolic compounds in the extracts.
The ability of green extract, ultrasonic extract and metanolic extract of S. sclarea to inhibit AChE, BChE and Urease enzymes were evaluated in this study for the first time.Our results proved the green extract showed as choline esterase (both AChE and BChE) inhibition better than conventional extracts including methanol and ultrasonic extract.Several studies have documented that the natural products and their derivatives can remedy Alzheimer's disease by inhibiting acetylcholinesterase enzyme (AChE) and butyrylcholinesterase enzyme (BChE) 57,58 .The essential oil extracted from Salvia lavandulaefolia and O. syriacum have shown significant anticholinesterase inhibitory activity 59,60 .Moreover the phenolic compounds obtained from Salvi species were found as considerable (AChE) and (BChE) inhibitory 61 , furthermore the phenolic compounds have previously described as AChE and BChE inhibitory molecules 62,63 .The inhibition of (AChE) and (BChE) observed in our investigation could be attributed to the presence of phenolic compounds detected already in extracts.Meanwhile, the results obtained from the urease inhibition assay showed the methanolic and ultrasonic extracts possess moderate urease inhibition however the green extract showed minimal inhibition against urease the enzyme.
The earlier studies have reported that phenolic compounds such as Gallic acid and Quercetin possess a great capacity to inhibit urease enzyme 64,65 , and although the compounds mentioned were present in extracts tested, however, the green extract showed little inhibition which can be attributed to the state of the extract, the other compounds in an extract could interfere with each other and the activity of a known compound may be masked by another in the mixture.Antioxidant agents play a vital part in food preservation via inhibiting oxidation processes and contributing to health promotion made by many food supplements, nutrients and functional food ingredients 66 .In the current investigation, antioxidant activity was evaluated by different methods including β-carotene-linoleic acid assay, DPPH • assay, ABTS •+ assay, CUPRAC assay and metal chelating assay.The free radical quenching abilities of extracts including methanolic, ultrasonic and green extracts of S. sclarea were performed by using DPPH and ABTS assays.DPPH is a stable free radical, it can be easily squelched, by an antioxidant agent which it loses this absorption by accepting an electron or a radical species 67 .In DPPH assay; we found the green extract and methanolic extract showed considerable potential in scavenging free radicals even higher than the standards used (BHA and α-tocopherol).Likewise, the ABTS tests used to determine hydrogen donation and chain-breaking capacity of the tested sample, we found that the methanolic and green extracts showed competitive ABTS radical scavenging capacity significantly greater than the references used.Meanwhile, The antioxidant capacity was also estimated using the carotene/linoleic acid bleach test, the activity test for neutralizing free linoleate radicals as well as free radicals that are shaped in the system and attack highly unsaturated β-carotene patterns 68 .As the results we mentioned above the green extract and methanolic extract had almost the same activity, whereat both extracts exhibited significant activity.The activity observed might be attributed to the presence of antioxidant agents in extracts such as phenolic compounds that could prevent the spread of beta carotene demolition by linoleate or any other radicals that may be shaped within the system.
Cu 2+ reduction is used for the determination of electron donation activity.The ability of methanolic, ultrasonic, and green extracts to reduce Cu 2+ to Cu 1+ were tested.All tested extracts showed activity to reduce Cu 2+ , however, the methanol and green extracts showed significant ability in reducing Cu 2+ to Cu 1+ even more than standers used, the ability to reduce Cu 2+ to Cu 1+ of extracts tested was in the order of methanolic extract > green extract > ultrasonic extract.
Frequently, the capacity of metal chelation is assessed by determining the chelating effect of antioxidants to ferrous ion.In the current investigation the capacity of ferrous ion chelating of methanolic, green extract, and ultrasonic extracts of S. sclarea was evaluated via assessing Fe(II) ferrozine test system, where the results indicated as EDTA equivalents.It is already mentioned in the results section that the green extract exhibited the highest activity among the extracts tested, followed by methanolic extract and ultrasonic extract.The results are in close agreement with the literature reported for Salvia species such as; Salvia virgata Jacq 69 , Salvia officinalis L. 70 , Salvia fruticosa 71 , and Salvia verticillata 72 to possess antioxidant properties substantially.
The antioxidant activity observed in this investigation could be linked to the existence of phenolic compounds abundantly which are demonstrated as antioxidant agents such as Quercetin 73 , Rosmarinic acid 74 and Gallic acid 75 .

Conclusion
Based on the results reported above the phenolic profile identified by HPLC-DAD revealed Rosmarinic acid was the major phenolic compound in all extracts.Ellagic acid was found only in the green extract, while 3-Hydroxy benzoic acid and Syringic acid, Ferulic acid, and Trans-cinnamic acid were detected in methanolic and ultrasonic extracts, while were absent in green extract.The green extract had better anti-biofilm activity than methanolic and ultrasonic extracts on S. aureus and E. faecalis; while methanolic extract had anti-biofilm activity than green and ultrasonic extracts on E. coli, C. albicans and P. aeruginosa PA01 at the same concentration, all extracts showed significant inhibition violacein production inhibition on C. violaceum CV12472, among the extracts tested, the ultrasonic extract showed as strongest inhibition of violacein production on C. violaceum CV12472, followed by green extract and methanolic extract, respectively.The green extract showed the highest swarming motility inhibition on P. aeruginosa PA01 better than methanolic extract and ultrasonic extract.The green extract exhibited good activity against BChE and AChE better than the methanolic and ultrasonic extracts, while ultrasonic extract and methanolic extract showed activity against urease enzyme, respectively better than the green extract.Furthermore, green and methanolic extracts showed considerable activity for β-carotene-linoleic acid, DPPH • , ABTS •+ and CUPRAC assay better than ultrasonic extract.However, green extract showed activity better than the methanolic and ultrasonic extracts in metal chelating assay.To the best of our knowledge the current investigation is the first paper demonstrated the therapeutical properties of S. sclarea by using green extraction.Moreover, the current study confirmed the efficiency of the green solvent in the extraction of bioactive compounds from natural sources as the new promising solvent, nontoxic cheaper, and friendly to the environment.

Figure 2 .
Figure 2. Structures of compounds identified in methanol ultrasonic and green extracts.

Figure 5 .
Figure 5. Quorum sensing inhibition zones in C. violaceum CV026 of )] of methanol, ultrasonic and green extracts of S. sclarea.

Table 1 .
Phenolic composition of the methanol extract , ultrasonic extract and green extract of S. sclarea by HPLC-DAD (µg/g).

Table 3 .
Anti-biofilm inhibition of methanol, ultrasonic and green extracts of S. sclarea.

Table 4 .
Quorum sensing inhibition zones in C. violaceum CV026 by test samples.

Table 5 .
Inhibition of violacein production in C. violaceum CV12472 by test samples.
Figure 6.Violacein production inhibition in C. violaceum CV12472 of methanol, ultrasonic and green extracts of S. sclarea.

Table 6 .
Swarming motility inhibition on P. aeruginosa PA01 by test samples.
Figure 7. Swarming motility inhibition on P. aeruginosa PA01 of methanol, ultrasonic and green extracts of S. sclarea.

Table 7 .
Cholinesterase and Urease inhibitory activities of the tested extracts.Values represent the means ± SEM of three parallel sample measurements (p < 0.05).NT= Not tested.